anticancer activity of polyisoprenoids from avicennia alba blume. in widr cells

Colorectal cancer is the third most common cancer world wide and has been occurred more in developing regions. the use of conventional chemotherapy agents may lead to various adverse effects. therefore, it is required to find the potential drug for anticancer from alternative source of natural product including mangrove plants. the present study was conducted to determine the anticancer activity of polyisoprenoids from avicennia alba blume. leaves (pal) in widr cells. cell cycle inhibition, apoptosis activity, and suppression of cyclooxygenase2 (cox2) were also evaluated. the anticancer activity of pal was determined by observing the activity of these compounds against widr cells using the [3(4,5dimetiltiazol2il) 2,5difenil tetrazolium bromida] mtt assay. inhibition of the cell cycle and increased apoptosis were analysed by flowcytometry. suppression of cox2 was analysed using immunocytochemistry. pal exhibited anticancer activity against widr cells with an ic50 of 173.775 μg/ml. cell cycle analysis revealed that the inhibition occurred in the g0g1 phase, and apoptosis occurred in the early apoptosis phase. furthermore, the result of an analysis of cox2 expression showed that pal enabled the suppression of cox2 expression. pal can be used as anticancer agents against widr colon cancer cells. however, invivo studies is required to confirm the invitro finding of the anticancer activity of polyisoprenoid extract.