Wedelolactone from Vietnamese Eclipta prostrata (L) L. Protected Zymosan-induced shock in Mice

Wedelolactone is known to have biological activities such as anti-inflammation hepatitis, anti-hepatotoxic activity, and trypsin inhibitory effect. however, up to date, there has not been any deep study on the role of wedelolactone for zymosan-induced signaling pathways in the process of regulating the excessive inflammatory responses in host. here, we demonstrated that wedelolactone plays an essential role for regulation of zymosan-induced inflammatory responses in murine bone marrow-derived macrophages (bmdms). the zymosan-mediated secretion of tumor necrosis factor-α (tnf)-α), interleukin (il)-6), and il12p40 but not il- 10 in bmdms was significantly inhibited by pre-treatment with wedelolactone (30 µg/ml, p < 0.001). furthermore, zymosan-induced supreoxide generation, nadph oxidase (p < 0.001), phosphorylation of p47phox in bmdms were significantly reduced by pre-treatment of wedelolactone (30 µg/ml). collectively, these data indicated that wedelolactone reduced zymosan-induced inflammatory responses. moreover, in-vivo wedelolactone (30 mg/kg) was significantly rescued from zymosan-induced shock through inhibition of systemic inflammatory cytokine levels.