Co-delivery of Sulforaphane and Curcumin with PEGylated Iron Oxide-Gold Core Shell Nanoparticles for Delivery to Breast Cancer Cell Line

Co-delivery approach has been recommended to reduce the amount of each drug and toachieve the synergistic effect for cancer treatment. curcumin (cur) and sulforaphane (sf) have antitumor effects, but their application is limited because of their low water solubility and poor oral bioavailability. to improve the bioavailability and solubility of sf and cur, we performed an innovative co-delivery of them with pegylated gold coated fe_3o_4 4magnetic nanoparticles (pegylated fe_3o_4 @au nps) to endorse sf and cur maintenance as an effective and promising antitumor drugs. the structure of the synthesized nanocarriers evaluated by x-ray diffraction, transmission electron microscopy, scanning electron microscopy, vibrating sample magnetometer, dynamic light scattering and fourier transform infrared spectroscopy. the results revealed that the zeta potential of cur and sf-loaded nps were about -15.4 mv and the average sizes were 80.57 nm. they were mono dispersed (polydispersity index = 0.161 ± 0.016) in water with high drug-loading capacity and stability. cur and sf were encapsulated into nps with loading capacity of 17.32 ± 0.023% and 16.74 ± 0.015% and the entrapment efficiency of 83.72 ± 0.14% and 81.20 ± 0.18% respectively. the in-vitro study of sf and cur loaded pegylated fe_3o_4 @au nps on human breast adenocarcinoma cell line3 4(mcf-7) confirmed that cytotoxicity of sf and cur can enhance when they are loaded on pegylated fe_3o @au nps in comparison to free sf and cur. the results of real-time pcr and flow cytometry shown that this combination can increase therapeutic effects of sf and cur by apoptosis and necrosis induction as well as inhibiting of migration in mcf-7 cell line.