Optimization and Characterization of Aspirin Encapsulated Nano-liposomes

Resistance to aspirin and its cytotoxicity significantly limits its therapeutic applications. nano- liposomal encapsulation of aspirin can reduce its cytotoxicity. in this study, aspirin encapsulating nano-liposomes (as-nl) was prepared and its performance in drug delivery and also cytotoxicity was evaluated. the effects of two independent variables including number of freeze/thawing cycles and concentration of aspirin on encapsulation efficiency was investigated using response surface methodology (rsm). a drug profile release was obtained by as-nl. the concentration of cholesterol as effective for liposome stability and sodium lauryl sulfate (sls) as a drug release facilitator was also optimized using rsm. the maximum aspirin encapsulation efficiency (41.44%) and drug release (33.92%) was obtained for 0.514 mg cholesterol and 0.007 mg sls used for liposome formulation. the morphology and size of as-nls were characterized using transmission electron microscopy (tem) and dynamic light scattering (dls). the stability of as-nl was evaluated by measuring the size change of nano- liposomes during 21 days using dls analysis. the stability of as-nl during this period was acceptable. the cytotoxicity test of as-nl by mtt test reveals the cytotoxicity of aspirin canbe reduced by using liposome encapsulation.