interleukin-37 relieves allergic inflammation in a house dust mite allergic rhinitis murine model

The purpose of this study was to investigate the effects of interleukin-37 (il-37) on a dermatophagoides farinae (der f)-induced murine model of allergic rhinitis (ar). balb/c mice, except the control groups, were sensitized intraperitoneally and challenged intranasally with der f (der f group). the il-37 and il-37+anti-cd25 groups were administered il-37 intranasally. the il-37+anti-cd25 groups were administered anti-cd25 monoclonal antibody intraperitoneally before challenge. allergic symptoms and the average eosinophil number were counted. the levels of cytokines and transcription factors in the nasal mucosa were measured by real-time polymerase chain reaction (pcr) and western blotting. the levels of der f-specific immunoglobulin e (ige) were measured. the cd4+cd25+foxp3+t cells among splenic mononuclear cells were analyzed by flow cytometry. the allergic symptom scores and der f-specific ige levels were lower in the il-37 group compared to the der f group. additionally, the levels of the transcription factor gata-3 and ror-γt and those of the cytokines il-4, il-5, il-13, and il-17, representing both t helper (th)2 and th17 responses, were lower in the il-37 group in comparison with the der f group. however, the th1 responsewas not suppressed after administration of il-37. il-37 increased the il-10 level; however, real-time pcr, western blotting, and flow cytometry results showed the limited action of il-37 on cd4+cd25+foxp3+t cells. this study demonstrates that intranasal il-37 can suppress th2 and th17 responses in an ar murine model. furthermore, these data suggest that il-10 is increased, but cd4+cd25+foxp3+t cells are not correlated with the il-37-induced mechanism.