Anti-Inflammatory Property of Β-D-Mannuronic Acid (M2000) on Expression and Activity of Matrix Metalloproteinase-2 and -9 Through Cd147 Molecule in Phorbol Myristate Acetate-Differentiated Thp-1 Cells

The aim of this study was to evaluate the effects of m2000, a novel non-steroidal anti-inflammatory drug (nsaid) with immunosuppressive property and without gastro-nephrotoxicitic effects on matrix metalloproteinases (mmp)-2 and (mmp)-9 in phorbol myristate acetate (pma)-differentiated thp-1 cells. gene expression and activity of mmp-2 and mmp-9 are inhibited respectively by the tissue inhibitor of matrix metalloproteinase (timp)-2 and (timp)-1 and are induced by extracellular matrix metalloproteinase inducer (cd147/emmprin). in this study, real-time quantitative reverse transcription polymerase chain reaction (rt-pcr) was used to determine gene expression of mmp-2, mmp-9, timp-1, and timp-2. flow cytometry and zymography were applied to determine cellular surface expression of cd147 and activity of mmp-2 and mmp-9, respectively. our results showed that treatment of thp-1 cells with high concentration (25 µg/ml) of m2000 significantly decreased the cellular surface expression of cd147 (p<0.05) and the gene expression of mmp-2, mmp-9 and timp-1 (p<0.05), and inhibited the gelatinolytic activity of mmp-2 and mmp-9 (p<0.05). according to our results, m2000 can reduce inflammation through inhibition of the cellular surface expression of cd147 and decrease the gene expression and gelatinolytic activity of mmp-2 and mmp-9 in pma-differentiated thp-1 cells.