The 14kDa Protein Molecule Isolated from Garlic Suppresses Indoleamine 2, 3- Dioxygenase Metabolites in Mononuclear Cells in vitro

A wide range of biological activities of garlic in vitro and in vivo have been verified includingits antioxidant, antitumor and anti-inflammatory effects. indoleamine 2,3-dioxygenase (ido) isan enzyme widely distributed in mammals and is inducible preferentially by ifn-gamma. idodegrades the essential amino acid tryptophan to form n-formyl kynurenine.in the present in vitro study, the modulatory effect of 14kda molecule isolated from garlicon ido induction was tested. cultures of mononuclear cells were exposed to 14kda garlicfraction. then, their proliferation responses and ido metabolites were measured.a significant down-regulatory effect of garlic on ido activity was found and also theproliferation responses of mononuclear cells increased.if these results are verified in vivo, an explanation will be provided on how garlic mayinterfere in ido induction, which paves the way for elucidating its specific therapeutic effectin preventing tumor progress.