Generation of Immune Inhibitory Dendritic Cells Andcd4+T Regulatory Cells Inducing By Tgf-Β

Variety of positive as well as negative regulatory signals are provided by antigenpresenting cell in particular by dendritic cells. in this research, we studied the capacity ofdendritic cells to expand antigen-specific t regulatory cells.we also investigated the role oftgf-beta in induction inhibitory functions of dendritic cells in mixed leukocyte reactions.dendritic cells were generated from blood cd14+ monocytes with granulocyte-monocytecolony stimulating factor and interleukin-4 with or without tgf-beta (tgf-β-gm-dc orgm-dc). cd4+ t cell were isolated to assess lymphocyte proliferation by lymphocytetransformation test assay and the ratio of cd4+foxp3+ cd25+ t cells were determined byfluorescene-activated cell sorter.t cell proliferation responses in gm–dc showed a significance antigen-specificproliferative response comparing with tgfβ-gm –dc. t cell proliferation was inhibited inco-culture system containing dc-treated tgf-β.it can be suggested that the expsansion of t regulatory by tgf-β-gm-dc provides ameans for antigen specific control of unwanted immune reactions.