Induction of Experimental Allergic Encephalomyelitis in C57/BL6 Mice: An Animal Model for Multiple Sclerosis

Basic research on the autoimmune disease multiple sclerosis has been performedmainly on its animal model namely experimental allergic encephalomyelitis. there aremany different approaches established to get this model. despite the existence of manyreferences in literature in this regard, we have been faced with many difficultiesgenerating the model suitable for studying different therapies. after a long time ofchallenging to get a reliable and replicable method, we came up with the following majorpoints: first, the key element for getting a maximum number of sick animals at a definedtime is to consider the most appropriate animal body weight (19-20 gr). even though theage of immunized animals (6-8 week old) is highlighted in literature, we found out thatbody weight is of a greater importance. secondly, because the only available susceptiblemice strain in iran is c57/bl6, the choice of peptide for immunization would be myelinoligodendrocyte glycoprotein (35-55 sequence of this peptide 200 ىg/animal). finally,pertussis toxin which is a costly reagent plays a key role in stimulating the immuneresponse. altogether, we recommend that considering the above mentioned tricks andtracks, one would definitely be able to generate a chronic progressive type of model, forbasic research on therapies of multiple sclerosis.