Association between the Polymorphisms of IL-4 Gene Promoter (-590C(GREATER THAN)T), IL-13 Coding Region (R130Q) and IL-16 Gene Promoter (-295T(GREATER THAN)C) and Allergic Asthma

Auergic asthma is a multifac to rial disease, influ en ced by ge netic and envi ronmentalfactors, recent famil y-b ased studies have revealed evidence for linkage o f humanchromosomes 5q31-33, 12q15-24, 11q13 and 15q23.6 as region s likely to contain genesrelat ed to as thma. among the ca ndida te genes in these regions arc the genes enco di ng fo rhuman int erleukin -4, int erleukin-l3 and interleukin-16.to evaluate thi s linkage, we examined an iranian population of patients with asthma . atotal o f 30 patients with allergic asthma and 50 normal subjects were studied, allergic asthmawas co nfirmed using skin prick test and spiro metry, dna was extrac ted from blood cellsand it a (-590c(greater tthan)t) , 11.-13 (r130q) and il-1 6 (-295t(greater tthan) c) pol yrnorphism s were determ inedby pcr-rflp method .o ut o f 30 patients with allergic as thma, the following genotypes for il-4, 1l-13 and 1l16cytokincs were fou nd: il-4 ge no types consisted of 17 (56.7%) cc , 8 (26.7°;(/) ct and 5(16.7'1.1) tt; il-13 geno types co ns isted o f 11 (36.7%) g g, 13 (43 ,3%) ga and 6 (20%) aa;il -16 geno types consisted o f 21 (76.7%) tt and 7 (23.3%) ct. no patient showed ccgenotype for il-l 6. a high er p roportion o f case subjec ts with th e c allele for the il-4, gallele for the il-l 3 and t allele for the 1r,-16 polymorphisms was found compared with th et , a and c alleles, res pe ctively.t hese results sugges t an influ en ce o f genetic var iability at th e promo ter of il-4 gene(-590c (greater tthan)t) and a coding region o f il-13 gene (r130q) o n the occurre nce o f allergic as thmaand no relationship between il-16 promote r polymorphism (-295t (greater tthan) c) and thi s di sease,