High Production of IL-18 by Dendritic Cells Induced by Sera from Patients with Primary Antibody Deficiency

Predominantly antibody deficiencies are a category of primary immunodeficiency diseases, whichconsist of several rare disorders such as common variable immunodeficiency (cvid) and x-linkedagammaglobulinemia (xla). we evaluated the effects of cvid and xla patients’ sera as a source ofmicroenviromental factors on maturation and function of monocyte-derived dcs.blood was collected from 10 cvid and 5 xla patients before immunoglobulin replacementtherapy and also from 8 healthy volunteers in order to obtain necessary sera for this study. monocytederived dcs were generated from blood cells obtained from healthy volunteers in the presence ofgm-csf, il-4 and 10% serum concentrations from cases and controls. immature dcs wereincubated with monocyte conditioned medium (mcm) and tnf-α in order to generate mature dcs.interleukin 18 (il-18) production by cd40l-activated mature dcs was measured after 24 hours ofculture in vitro.il-18 production by dcs generated in the presence of cvid and xla patients’ sera were6.75±2.59 and 7.08±1.75 ng/ml, respectively, which were significantly higher than normal serumconditioned dcs (3.55±0.68) ng/ml.these results suggest that the sera of patients with predominantly antibody deficiencies maycontain soluble factor(s) that can induce a significant increase in il-18 production by dcs