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The Effect of Vitamin E on Splenocytes Apoptosis of Gamma-Irradiated BALB/c Mice
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نویسنده
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Maroufi Dahman ,Sussan Ardestani Kaboudanian ,Karlminia Amina ,Naderimauesh Hussain
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منبع
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iranian journal of allergy, asthma and immunology - 2005 - دوره : 4 - شماره : 2 - صفحه:77 -82
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چکیده
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Apoptosis, a physiologic mechanism to eliminate unwanted cells, is also induced byionizing irradiation, through production of free radicals. it has been demonstrated thatantioxidants such as vitamin e are able to protect cells from damage caused by freeradicals. taken together we found it reasonable to make an attempt to evaluate theprotective effect of vitamin e against apoptosis. the irradiated mice received 1 gy/daygamma radiation for one day (low dose) or for three successive days (high dose, 3gy).the splenocytes were then isolated at 6, 14 and 24 h after exposure. dna gelelectrophoresis and dna fragmentation assay were done in addition to the evaluationof splenocytes cytology.our results showed that vitamin e can reduce apoptosis against low doseirradiation. however it is not able to completely block programmed cell death in highdose irradition.
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کلیدواژه
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Apoptosis; Free Radicals; Gamma Radiation; Vitamin E
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آدرس
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university of tehran, Institute of Biochemistry and Biophysics, ایران, university of tehran, Institute of Biochemistry and Biophysics, ایران, Pasteur Iinstitute of Iran, Department of Immunology, ایران, tarbiat modares university, Faculty of Science, Biophysics Department, ایران
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پست الکترونیکی
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ardestani@ibb.ut.ac.ir
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Authors
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