IL-l, IL-IR and TNFa Gene Polymorphisms in Iranian Patients with Multiple Sclerosis

Different research groups have extensively studi ed the associatio ns of cytokine genepolymorphisms in different diseases. the role o f cytokines gene polymorphisms in multiplesclerosis (n1s), as a chronic immune-mediated neurodegenerativ e disease, has beenpreviou sly reported in the various populations.for determining pro -inflamm atory cytokine gene polymorphisms, 100 relapsing remittingmultipl e sclerosis (rrms) iranian pati ents and 140 normal individuals as control enrolled inthis study. dna of each sampl e was extracted by a modified salting ou t method . cytokinesingle gene nucleotide polyrnorphisms including il-1ix -889, il-1~ (-511 and +3962), il-1rpst1 1970, il-1ra mspal111 00, and tnf-ix (-308 and -238) were det ermined by using thepcr-s sp meth od .the resul ts of our data indicate the dec rease in frequency of il-1ix tc-889 genotype(p=0.002), il-1~ tc +3962 genotype (p=0.004), il-1r t pst1 1970 allele (p= 0.0001), il-1ra tc mspa1 11100 genotype (p=0.009), tnf-ix a-308 allele (p=0.0002) and ag genotype(p=0.00001) in the patients group ver sus normal subj ects. on the other hand the frequ encyof il-1ix it -889 genotype (p=0.028), il-1r c pst1 1970 allele (p= 0.0001) and cc genotype(p=0.00006), tnfix g -308 allele (p=0.0002) and gg geno type (p=o.oooool) decre asedsignificantly in the pati ents versu s normal subj ects.these result s suggest that polymorphic variations of these pro-inflammatory cytokinesmay play an important role in susceptibility of ir anian multiple sclero sis patients.