Co-Administration of CpG Oligonucleotides and Chenopodium album Extract Reverse IgG2a/IgGl Ratios and Increase IFN-Gamma and IL-IO Productions in a Murine Model of Asthma

Asthma is a disorder of increasing severity and prevalence. recent knowledge about thepathogenesis of asthma emphasizes its inflammatory nature. cpg oligonucleotides are a classof compounds containing motifs based on the cytosine-guanine dinucleotides (cpg-odns).these motifs are suppressed in mammalian dna. they induce inflammation in mammalscharacterized by the induction oft helper type 1 and regulatory responses.in this paper, the effect of cpg dna co-administration with a homemade chenopodiumalbum (ch.a) extract in a murine model of asthma is reported for the first time . balb/c micewere sensitized using ch.a. pollen allergenic extract plus cpg-odns intraperitoneally andwere challenged with aerosolized allergen. results measured included il-i0 and ifn-gammacytokines as well as igg subclasses. for this, splenocytes from mice treated with cpg / ag orag alone, were cultured in the presence of antigen.the results showed that cpg odn administered at the time of ch.a sensitization,effectively increased cytokines and igg2a/lgg1 ratios compared with those in mice treatedwith antigen or with pbs alone(ps 0.001). our experiments revealed that ch.a. sensitizationdecreased igg2a/lggl compared with non-sensitized mice (ps 0.001), while cpgodn/ch.a reversed this ratio, indicating cpg potentials towards igg2a subclass switching.we conclude that co- administration of ch.a. allergen and cpg odn prevents thedevelopment of th2-mediated response probably through the il-i0 regulatory effects.thus, these components could be used with the other allergens in order to induce theprevention of inflammatory conditions. we suggest furth er studies are neces sary to identifythe potential effects of cpg-odns administration in conjunction with other antig ensprepared from the regional allergens in iran. taken together, we suppose that the resultsobtained in this study in animal models may be useful in human trials conducted by otherinves tigators .