The Effect of IL-22 and IL-28 in Induction of Type 1 Regulatory T (Tr1) Cells

Cytokines have been introduced as critical inducers in the development of th subpopulations.cytokines like il-10 are involved in inducing regulatory t cells such as type 1 regulatory t (tr1) cells cells. il-22 is a member of il-10 family of cytokines, and il-28a is a member of ifn-γ family. in this study, cord blood mononuclear cells (cbmc) from normal healthy individuals were isolated by ficoll and then naïve t cells were purified by cd4+cd25+ regulatory t cell isolation kit. the effect of these two cytokines on production of il-5, tgf-β, il-10, il-4 and ifn-γ cytokines from cord blood t cells was investigated to identify tr1 cells as well as th1 and th2 polarization. flow cytometric analysis showed that il-28a and il-22 were not effective in expression of il-5 and tgf-β either alone or in synergy, but in view of il-10, il-4 and ifn-γ, the results showed that il-22 increased il-10 and il-4 but had a decreasing effect on ifn-γ. the results showed that il-28a was not effective in increasing or decreasing the level of il-10, il-4 and ifn-γ. therefore, according to these results, il-22 and il-28a were not effective in inducing tr1 cells.