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Development of a New Immunochromatographic Assay Using Gold Nanoparticles for Screening of IgA Deficiency
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نویسنده
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Goudarzi Saeid ,Ahmadi Anita ,Farhadi Mohammad ,Kamrava Kamran ,Saghafi Shiva ,Omidfar Kobra
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منبع
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iranian journal of allergy, asthma and immunology - 2015 - دوره : 14 - شماره : 1 - صفحه:105 -112
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چکیده
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A new competitive immunochromatography (icg) strip test based on polyclonal antibody (pab) conjugated with gold nanoparticles (nps) was developed and its applications for primary screening of immunoglobulin (ig) a in serum were evaluated. nanocolloidal gold as the detection reagent, with an average particle diameter of 20 nm, was synthesized and labelled pab. the antibody-nanocolloidal gold probe was applied on the conjugate pad, and human iga was immobilized on a nitrocellulose membrane as the capture reagent to prepare the icg strip test. it took only 10 minutes to accomplish a semi-quantitative detection of serum iga in this assay.in the optimized investigational conditions, the icg strip test could distinguish human serum iga in the range from 1 to 270 ng/ml with a detection limit of 5 ng/ml. the reliability of testing procedures was examined by performing the icg strip test with 11 serum samples and comparing the results with those obtained via enzyme-linked immunosorbent assay (elisa). the icg strip was sufficiently sensitive and accurate for a rapid screening of iga in human serum.
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کلیدواژه
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IgA deficiency; Immunoglobulin A; Immunochromatography; Gold nanoparticle; Screening test
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آدرس
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iran university of medical sciences, Hazrate Rasoul Akram Hospital, ENT- Head & Neck Research Center, Clinical Nanomedicine Laboratory, Tehran, tehran university of medical sciences tums, Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, ایران, iran university of medical sciences, Hazrate Rasoul Akram Hospital, ENT- Head & Neck Research Center, Clinical Nanomedicine Laboratory, ایران, iran university of medical sciences, Hazrate Rasoul Akram Hospital, ENT- Head & Neck Research Center, Clinical Nanomedicine Laboratory, ایران, tehran university of medical sciences tums, Immunology, Asthma and Allergy Research Institute, ایران, tehran university of medical sciences tums, Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, ایران
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پست الکترونیکی
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omidfar@tums.ac.ir
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Authors
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