Interleukin-25 Enhances Allergic Inflammation through p38MAPK and NF-κB Pathways in Mouse Models of Allergic Rhinitis

Interleukin (il)-25, a cytokine of il-17 family, can activate p38 mitogen-activated protein kinases(mapk) and nuclear factor(nf)-χb pathways to propagate th2 responses. the allergic rhinitis mouse model was established by stimulating balb/c mouse with ovalbumin (ova). then we detected expression of il-25 and downstream p38mapk and nf-χb. the expression of il-25, p38mapk and nf-χb were detected in the ova-induced allergic rhinitis mouse model. the allergic parameters, such as allergic symptoms, serum ova-specific immunoglobulin e (ige) levels and eosinophil infiltration in the nasal mucosa were compared between ova group and control group. ova-induced mice displayed significantly higher allergic responses compared with the saline control group. ova induced mice demonstrated more allergic symptoms, higher serum ova-specific ige levels and eosinophil infiltrations. the increased expression of il- 25, p38mapk and nf-χb immunoreactivity were detected in epidermal cells in the ova group. the mrna measurement of il-25, p38mapk and nf-χb showed the same result. il-25 enhances the ova-induced allergic rhinitis by activating p38mapk and nf-χb pathways.