Effect of Toll-Like Receptor 3 Agonists on the Functionality and Metastatic Properties of Breast Cancer Cell Model

There exists compelling evidence that toll-like receptor 3 (tlr3) agonists can directly affect human cancer cells. the aim of this study was to investigate anti-cancer effects of tlr3 agonist in human breast cell line. we assessed potential effects of poly (a:u) on human breast cell line (mda-mb-231) on a dose-response and time-course basis. human breast cell line mda-mb-231 was treated with different concentrations of poly (a:u) and lipopolysaccharide (lps). then, the following assays were performed on the treated cells: dose-response and time-course cytotoxicity using colorimetric method; matrix metalloproteinase-2 (mmp-2) activity using gelatin zymography method; apoptosis using annexin-v flowcytometry method; and relative expression of tlr3 and mmp-2 mrna using reverse transcriptase polymerase chain reaction (rt-pcr) method. following treatments, doseresponse and time-course cytotoxicity using a colorimetric method, (mmp-2) activity (using gelatin zymography), apoptosis (using annexin-v flowcytometry method) assays and expression of tlr3 and mmp-2 genes (using pcr method) were performed. cytotoxicity and flowcytometry analysis of poly (a:u) showed that poly (a:u) do not have any cytotoxic and apoptotic effects in different concentrations used. mmp-2 activity analysis showed significant decrease in higher concentrations (50 and 100 μg/ ml) between treated and untreated cells. moreover, poly a:u treated cells demonstrated decreased expression of mmp-2 gene in higher concentrations. collectively, our data indicated that human breast cancer cell line (mda-mb-231) was highly responsive to poly (a:u). the antimetastatic effect of direct poly (a:u) and tlr3 interactions in mda-mb-231 cells could provide new approaches in malignant tumor therapeutic strategy