evaluation of t-cell function after blood transfusion in patients undergoing coronary artery bypass grafting

Blood transfusion is associated with increased mortality and morbidity. this study aimed to determine the effect of blood transfusion on t-helper 1 (th1), th2, and th17 function in patients undergoing coronary artery bypass grafting (cabg).two blood samples were obtained from patients undergoing cabg, before and 14 days after surgery. production of interleukin (il)-2, il-4, interferon (ifn)-γ, il-17a, and il-10 by cd4+ t cells was measured using enzyme-linked immunosorbent assay (elisa). mrna expression of t-box expressed in t cells (t-bet), gata binding protein 3 (gata3), rar-related orphan receptor-γ (ror-γt), signal transducer and activator of transcription 3 (stat3), stat4, and stat6 were measured using quantitative reverse transcriptase polymerase chain reaction (qrt-pcr).mrna expression of t-bet and stat4 showed a significant decrease after blood transfusion. however, the concentration of ifn-γ in the culture supernatant showed no significant difference after blood transfusion. mrna expression of gata3 and stat6 showed a significant decrease after blood transfusion. however, the concentration of il-4 in the culture supernatant showed no significant difference after blood transfusion. mrna expression of ror-γt showed no significant decrease after blood transfusion; however, the expression of stat3 and the concentration of il-4 in the culture supernatant did significantly decrease following blood transfusion. il-10 production increased significantly postoperatively.decreased th1, th2, and th17 signaling pathway activity and increased il-10 concentration indicate an immunomodulatory effect on the immune system after blood transfusion.