microrna-486-3p targets chymotrypsin c to regulate pancreatic cancer progression and immunosuppressive factor expression

Pancreatic ductal adenocarcinoma (pdac) is a common digestive system tumor with high mortality rates and a poor prognosis. reports suggest that microrna (mir)-486-3p in pdac can be used as a diagnostic biomarker. this research aimed to elucidate the mechanisms by which mir-486-3p regulates pdac progression.mir-486-3p and chymotrypsin c (ctrc) expression in pdac were measured using quantitative real-time polymerase chain reaction. changes in the biological properties of pdac cells were assessed by transwell assay, scratch-wound assay, cell counting kit (cck)-8 assay, and plate cloning assay. the protein expression of immunosuppressive factors (vascular endothelial growth factor, interleukin-6, and transforming growth factor-β) in pdac cells was detected by western blot. additionally, a subcutaneous graft tumor model was constructed to explore the influence of silencing mir-486-3p on pdac in vivo.pdac showed a pronounced increase in mir-486-3p expression. upregulation of mir-486-3p stimulated pdac cell proliferation, migration, invasion, and immunosuppressive factor protein expression, whereas silencing mir-486-3p hindered pdac malignant development. mir-486-3p targets and negatively regulates ctrc expression. silencing ctrc partially rescued the restraining impact of silencing mir-486-3p on pdac malignant progression. in vivo experiments also indicated that silencing mir-486-3p inhibited pdac malignant progression and immunosuppressive factor expression in vivo.in summary, mir-486-3p promotes immunosuppressive factor protein expression by targeting and negatively regulating ctrc expression, which in turn promotes pdac malignant progression.