Effects of MnTnHex-2-PyP on Lung Antioxidant Defence System in Asthma Mice Model

We aimed to study the mntnhex-2-pyp effect on some markers of lung antioxidant defence system in mice asthma model.the study was carried out on 28 c57b1/6 mice divided into four treatment groups: group 1 – controls; group 2 – injected and inhaled with ovalbumin; group 3 – treated with mntnhex-2-pyp and inhaled with phosphate buffered saline; group 4 – injected with ovalbumin and mntnhex-2-pyp but also inhaled with ovalbumin. on days 24, 25 and 26, mice from groups 1 and 2 were inhaled with pbs for 30 min, and those from groups 2 and 4 were given a 1% ovalbumin solution. one hour before inhalation, and 12 hours later the animals from groups 1 and 2 were injected i.p. with 100 μl pbs, and those from groups 3 and 4 received a 100 μl mntnhex-2-pyp solution in pbs, сontaining 0,05mg/kg. the animals were killed by exsanguination 48 hours after the last inhalation for obtaining a lung homogenate.the activities of superoxide dismutase, catalase, glutathione peroxidase and the non-protein sulphhydryl group content in the lung homogenate were investigated. ovalbumin decreased the activities of superoxide dismutase (p=0.01), catalase (p=0.002), glutathione peroxidase and non-protein sulphhydryl groups content (p<0.001) in comparison to controls. in group 4 (ovalbumin and mntnhex-2-pyp) the activities of superoxide dismutase (p=0.044), catalase (p=0.045), glutathione peroxidase (p=0.002), and the non-protein sulphhydryl groups content (p<0.001) were significantly increased compared to ovalbumin (group 2).mntnhex-2-pyp restored the activities of basic enzymes in the lung antioxidant defence system in ovalbumin-induced asthma mice model, 48 hours after the last nebulization