evaluating the microrna expression of il-35 and il-37 in helicobacter pylori-infected patients with gastritis and gastric ulcer

Interleukin (il)-35 and il-37 are two anti-inflammatory cytokines. il-35 inhibits the development of t-effector cells such as th1, and th17; while increasing regulatory t cells (tregs). il-37 causes the suppression of inflammatory cytokines. regarding the positive impact of helicobacter pylori (h. pylori) infection on inflammation and considering the anti-inflammatory effects of il-35 and il-37, this study aimed to evaluate the expression of these two cytokines in h. pylori-infected patients with gastrointestinal problems. the case group consisted of h. pylori-infected individuals with gastric ulcer and/or gastritis (n=50) and the control group consisted of cases with gastric ulcer and/or gastritis non-h. pylori-infected (n=50). sampling and classification of patients were based on pathology findings. a real-time polymerase chain reaction was performed for evaluating the il-35 and il-37 expression levels. h. pylori-infected gastritis patients showed lower expression of il-35 and il-37 than the non-infected group. there was a significant difference between the expression levels of il-35 and il-37 in patients with gastric ulcers and/or gastritis who were infected and non-infected by h. pylori. there were no significant differences in the expression level of il-35 and il-37 in h. pylori-infected patients with gastric ulcer or gastritis. interleukins 37 and 35 were less expressed in patients with h. pylori-infection. in differentiation between patients with gastrointestinal symptoms who have h. pylori infection or with similar symptoms who do not have h. pylori-infection, mentioned interleukins can be used as diagnostic markers.