Evaluation of the Effect of IL-22 on Human Cord Blood CD4+ T Cells

Il-22 is a member of il-10 cytokine family which is believed to play an important role in inflammatory responses. il-22 has similarities with il-10 including conserved sequences with il-10. il-22 receptor is also comprised of two chains known as l-22r1 and l-10r2; supporting the speculation that the two cytokines may have similar effects. the aim of this study was to shed some light on the biological activity of il-22 upon the cord blood cd4+cd25- t cells. in this research, cord blood t cd4+cd25- cells were cultured in presence of anti cd2/cd3/cd28 coated beads, il-2 and il-22 for two weeks at 37 oc and 5% co2. flow cytometry analysis showed that il-22 has no effect upon cd25 and foxp3 expression. also, the results indicated that il-22 is not involved in cd4+ t cell proliferation. moreover, the results of suppression assay did not show any suppression effect on the cultured t cells. thus, it seems that umbilical cord blood t cells probably do not express il- 22r1 on their surface.