glucocorticoid receptor nuclear translocation in cd4 t cells from severe and moderate asthmatic patients treated with fluticasone/vilanterol

Different phenotypes of asthma from mild to severe are categorized based on diverse clinical features. a guideline for the recognition and treatment of asthma has been provided by global initiative for asthma (gina). to control symptoms and prevent asthma exacerbation in most patients combinational therapy with inhaled corticosteroids (ics) and a long acting b2-adrenreceptor agonist (laba) are recommended. understanding asthma phenotypes would be helpful to improve asthma diagnosis and treatment. the aim of this study was to verify glucocorticoid receptor glcococorticoid receptor (gr) nuclear translocation in cd4 t cells treated with fluticasone furoate (ff), vilanterol (v) and ff/v combination in severe asthmatic patients compare to patients with moderate asthma and healthy controls using immunocytochemistry (icc). after taking blood and separating pbmcs from each subject, cd4 t cells were isolated from pbmcs using cd4+ t cell isolation kit. isolated cd4 t cells were cultured in presence of ff, v and ff/v combination for 1 hour and after cytocentrifugation, cells were incubated with anti gr-antibody and subsequently stained with fitc bound secondary antibody and gr nuclear translocation was observed under microscope. the results showed significant increasing in gr nuclear translocation in treated cd4 t cells from patients with moderate asthma and controls compare to those severe asthmatic patients, along with treating cells with ff/v combination no significant gr nuclear translocation was observed compare to that of using mono treatment of cells with ff and v. based on our findings, it can be concluded different mechanisms are responsible for severe asthma and moderate asthma.