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development of methemoglobin-based biological dosimetry in gamma–irradiated mice
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نویسنده
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zhang x.h. ,zhang y.n. ,min x.y. ,lou z.c. ,wang a.l. ,hu x.d. ,zhang h.q.
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منبع
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international journal of radiation research - 2015 - دوره : 13 - شماره : 3 - صفحه:235 -241
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چکیده
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Background: a new biological dosimeter based on methemoglobin level was developed in this study. materials and methods: methemoglobin level in erythrocytes from mice subjected to γ rays from a 60co source was detected using the methemoglobin kit. the dose range was from 0.5 to 8 gy and the dose rate was 0.5 gy/min. results: the results demonstrate that methemoglobin level increases with increasing dose. the detection limit based on methemoglobin has a lower limit of dose estimation of about 1 gy. the high levels of methemoglobin are maintained for at least 28 days, and the maximal increase of methemoglobin observed occurs at about 30 min after γ irradiation. the relationship between dosage and the increased methemoglobin level can be expressed by a linear quadratic equation of y = -8.75 x^2 + 168.09 x + 32.66, with the correlation coefficient, r, equal to 0.96. the best suggested time for blood collection is up to 1 day after gamma irradiation. the doses absorbed by mice as estimated from the use of the dose-response relationship were close to the blind doses of 1, 2, 4 and 8 gy. conclusion: methemoglobin is a quick, simple, and precise biomarker for the early assessment of the absorbed dose in mice.
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کلیدواژه
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biological dosimetry ,methemoglobin ,gamma irradiation ,mouse
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آدرس
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nanjing university of aeronautics and astronautics, department of nuclear science and engineering, china, nanjing university of aeronautics and astronautics, department of nuclear science and engineering, china, nanjing university of aeronautics and astronautics, department of nuclear science and engineering, china, nanjing university of aeronautics and astronautics, department of nuclear science and engineering, china, bengbu medical college, first affiliated hospital bengbu medical college, china, nanjing university of aeronautics and astronautics, department of nuclear science and engineering, china, nanjing university of aeronautics and astronautics, department of nuclear science and engineering, china. southeast university, jiangsu laboratory for biomaterials and devices, china
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پست الکترونیکی
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zhanghq@nuaa.edu.cn
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Authors
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