LINC01204 Negatively Regulates the Effect of MiR-214 on Lung Cancer Cell Apoptosis, Migration, Invasion and Radiosensitivity

Background: to investigate whether lncrna linc01204 affects lung cancer cell apoptosis, migration, invasion and radiosensitivity by regulating mir-214. materials and methods: normal lung cells hbe and lung cancer cells a549 were cultured in-vitro, and pcdna3.1, pcdna3.1-linc01204, pcdna3.1-linc01204 and mir-nc, pcdna3.1- linc01204 and mir-214 mimics were transfected into a549 cells, respectively. qrt-pcr, flow cytometry, scratch test, and transwell chamber test, clone formation test, dual luciferase, and western blot methods were used in this study. results: compared with hbe cells, a549 cells had significantly reduced linc01204 expression level (p<0.05), and significantly increased mir-214 expression level (p<0.05); transfection of pcdna3.1-linc01204 could significantly increase apoptosis rate and cleaved-caspase3, e-cadherin protein levels (p<0.05), reduce migration healing rate, n-cadherin protein levels and cell survival fraction (p<0.05) with sensitization enhancement ratio ser at 1.950, reduce the number of invasive cells (p<0.05). dual luciferase report experiments confirmed that linc01204 can bind to mir-214 in a targeted way; co-transfection of pcdna3.1-linc01204 and mir-214 mimics can significantly reduce the apoptosis rate and cleaved-caspase3 and e-cadherin protein levels (p<0.05), improve the migration healing rate, n-cadherin protein level and cell survival fraction (p<0.05) with sensitization enhancement ratio ser at 0.728, increase the number of invasive cells (p<0.05). conclusion: linc01204 overexpression can negatively regulate mir-214 expression to promote lung cancer cell apoptosis and inhibit migration and invasion, thereby enhancing radiosensitivity.