Effects of adrenomeduline and ramp2 on the lung of mice exposed to total body radiation

Background: adrenomedullin (am) and its receptor, receptor activity- modifying protein (ramp) 2 have pleiotropic regulatory functions in normal tissue and cancer tissue. am is produced and secreted both numerous stromal cells and tumor cells. this study aims to investigate a possible role of am and ramp2 in the radiation exposure in the normal lung tissue. materialsand methods: four groups with 6 male adult swiss albino mice per groupwere investigated. the mice were subjected to a 500 cgy single -doseradiation exposure in the total body radiation device and lung tissues werecollected. 1, 2, and 7 days after radiation exposure, with 1 reference groupwhich was not exposed to radiation. results: the general histology and theimmunohistochemistry of the tissue samples prepared with anti- am, anti – ramp2, and monoclonal antibodies were investigated, yielding a statisticallysignificant increase for am on day 3 and for ramp2 on day 1 after radiationexposure. conclusion: the observed increase of am and ramp2concentrations in the normal tissue matrix after radiation exposure may playa role in the side effects of radiotherapy.