Radiosensitization of [Ru(bpy)2(phen)]Cl2 on nasopharyngeal carcinoma cells

Background: to investigate effect of radiosensitization of [ru(bpy)2(phen)]cl2 complex on nasopharyngeal carcinoma cell line cne1 and its mechanism.materials and methods: nasopharyngeal carcinoma cell line cne1 in vitroculture was divided into control group, light irradiation group (4 gy, 6 mvphotonic line), simple metal ruthenium complex treatment group (ru group,100 μmol/l [ru(bpy)2(phen)]cl2) and metal ruthenium complex combinedwith radiotherapy group (combined radiotherapy group, cells were irradiatedwith 4 gy and 6 mv photons at 2 days after 100 μmol/l [ru(bpy)2(phen)]cl2).results: transcriptional level of p53 gene in combined radiotherapy groupwas higher than that in the other groups (p<0.001). inhibition rate of combined radiotherapy group was higher than that of ru group andirradiation group (p<0.001). apoptotic rate was the highest (p<0.05) in thecombined radiotherapy group, and irradiation group was higher than rugroup and control group (p<0.05). survival rate of ru group was lower thanthat of control group under the same radiation dose (p<0.05), and theradiotherapy sensitization ratio was 1.227 (dq ratio). conclusion: [ru(bpy)2(phen)]cl2 increases the sensitivity of nasopharyngeal carcinoma cell linecne1 to x-ray, which may be related to increase of p53 gene expression.