Caveolin-1 was involved in the reduced adiosensitivity to X-ray in human mammary epithelial MCF10A cells

Background: x-ray chest fluoroscopy is a compulsory component of thehealth examination procedure in china. the radiation dose from chestfluoroscopy is the largest in x-ray examination. more than half of the womenin their twenties with breast cancer have been given x-ray fluoroscopy.studies have shown that caveolin-1 is involved in the repair of damage dnain tumor cells induced by irradiation. however the mechanism and role ofcaveolin-1 in normal human mammary epithelial cells are not clear.materials and methods: here, normal human mammary epithelial cells(mcf10a) and the cells with caveolin-1 knockdown (mcf10ace) were exposedto x-ray radiation to investigate the role of caveolin-1 in the enhancement ofradiosensitivity in these cells and the associated mechanism. results:decreased survival rate and a significantly higher level of cell arrest at the g1 and g2 phases, as well as reduced activation of the dna damage repair proteins atm and p53, and the stress protein p38mapk were manifested by mcf10ace cells compared to mcf10a cells, following exposure to x-ray radiation. furthermore, binding between caveolin-1 and mdm2 in mcf10acecells was also lower than in mcf10a cells. conclusion: overall, the finding indicated that caveolin-1 played an important role in decreasing the radiosensitivity of human mammary epithelial cells.