Diethyl Phthalate Causes Oxidative Stress: An in Vitro Study

Background: phthalates are a group of multifunctional chemicals. diethyl phthalate (dep) is one of the most frequently used phthalates in solvents and fixatives for numerous industrial products. method: the present experiment was designed to assess oxidative stress, if any, caused by diethyl phthalate. for this the homogenates of liver and kidney were treated with different concentrations ( 10-40 µg/ml) of dep. 10% liver and kidney homogenates were prepared in phosphate buffered saline and used for estimation of lipid peroxidation.in final step lipid peroxidation and total protein content were analyzed. results: the result revealed significant and dose - dependent increase in lipid peroxidation, whereas protein content reduced significantly. maximum increase in lpo and decrease in protein content was observed at 40 µg/ml of dep concentration. conclusion: from this study, it can be concluded that different concentrations of dep leads to dose- dependent significant increase in lipid peroxidation and decrease protein content.so at the different concentration of dep cause oxidative stress.