The Effect of Rebadioside A on Attenuation of Oxidative Stress in ‎Kidney of Mice under Acetaminophen Toxicity

Background: acetaminophen (apap) overdose causes renal and hepatic injury. it is ‎also believed that oxidative stress has a pivotal role in apap-induced renal injury. ‎therefore, protective effects of different antioxidants have been examined in apap-‎induced renal and hepatic toxicity models. stevia rebadiana is a plant with a high ‎degree of natural antioxidant activity in its leaf extract. the aim of this study was to ‎investigate the possible protective effects of rebadioside a; one of the main components ‎of stevia extract, on apap-induced oxidative stress in kidney of mice. ‎methods: oxidative stress was induced in kidney of balb/c mice by the intraperitoneal ‎‎(i.p.) administration of a single dose of 300 mg/kg apap. some of these mice also ‎received rebadioside a (700 mg/kg) (i.p.) 30 minutes after apap injection. two and six ‎hours after apap injection, all mice were sacrificed and malondialdehyde (mda), ‎glutathione (gsh), free apap, and glutathione conjugated of apap (apap-gsh) were ‎determined in the kidney tissue.‎results: gsh depletion and mda levels significantly (p < 0.05) increased in mice treated ‎with either apap or apap plus rebadioside a, respectively in 2 and 6 hours intervals ‎after apap administration. significantly (p < 0.05) higher levels of free apap and ‎apap-gsh levels detected in kidney of mice administrated with apap plus rebadioside ‎a compared to apap treated ones.‎conclusion: rebadioside a may be a potential compound in alleviation of apap-‎induced oxidative stress in kidney of mice after apap overdoses. ‎