flavonoid-rich buckwheat extracts as natural modulators of sirtuins and anti-toxic agents

Background: polyphenolic compounds in buckwheat (fagopyrum esculentum) are known for their antioxidant and detoxifying properties. among them, rutin is a major flavonoid with potential biological activity. the present study focused on the comparative analysis of polyphenolic content in roasted and green buckwheat and explored rutin’s interaction with sirtuin (sirt), a key protein involved in cellular stress responses and longevity. methods: high-performance liquid chromatography (hplc) was employed to quantify polyphenolic compounds in roasted and green buckwheat samples. in silico molecular docking was performed using cb-dock2 to assess the binding affinity of rutin with the active site of sirt. results: the hplc results demonstrated that green buckwheat contained significantly higher levels of rutin compared to roasted buckwheat (1.09 mg/g vs. 0.5 mg/g). molecular docking revealed a strong binding affinity between rutin and sirt, supporting its potential role in modulating sirtuin-related protective pathways involved in detoxification. conclusion: green buckwheat is a richer source of rutin, which may contribute to its enhanced detoxification potential. the strong in silico interaction between rutin and sirt2  suggests that rutin could serve as a natural modulator of cellular defense mechanisms, particularly those regulated by sirtuins.