in silico profiling, acute toxicity study, and haematological analysis of chlorogenic acid in swiss mice

Background: chlorogenic acid (cga) is a phytonutrient, widely distributed in diet and taken as a food supplement, and has been shown to possess various health benefits. however, in silico investigation and the effect on haematological parameters, especially in the light of acute ingestion, is scanty. the present study aimed to unravel, through predictive modeling, the effects of cga (chlorogenic acid) in biological systems and assess its toxic effects in the context of acute exposure. this was achieved by conducting observations and analyzing haematological parameters as a preliminary approach.methods: chemical absorption, distribution, metabolism, excretion, and toxicity (admet) was employed for the in silico profiling using admetsar2.0. acute toxicity study was conducted by weight-matching ninety-six male swiss mice into eight groups (n=12) and exposed to distilled water (control), 1% ethanol (vehicle), and six doses of cga (30, 60, 120, 240, 480, and 1,000 mg/kg body weight). after 24 h, the animals were observed for mortality; six of the animals were bled, and blood samples were analyzed for haematological parameters using an autohaematology analyzer. the animals that survived were observed for 14 days post-treatment to monitor for mortality and signs of toxicity.results: the admet profile predicted that cga had a tendency towards hepatotoxicity and toxicity in the respiratory and reproductive systems but no effect on the haematological investigation, except at the 1000 mg/kg dose. it is noteworthy that one animal died at the 60 mg/kg dose.conclusion: the cga may be considered tolerable when its target organs are not the reproductive and respiratory systems or the liver, while being deemed safe concerning haematological parameters.