Effect of Nano-Eugenol and Aerobic Exercise Against the StreptozotocinToxicity and Inflammatory Mediators P38-Mapk, Npy, and A-Rα2a in the Dorsal Root Ganglia of Diabetic Rats

Background: the aim of this study was to investigate the effects of nano-eugenol combined with aerobic exercise against the streptozotocin toxicity and inflammatory mediators p38- mapk, npy and a-rα2a in the dorsal root ganglia of diabetic rats. methods: twenty-five, 8-week-old wistar male rats were divided into 5 groups: 1) normal control group (normal model); 2) diabetic control group (diabetic model); 3), diabetic + exercise group (diabetic+exercise model); 4) diabetic group + nano-eugenol (diabetic+nano model); and 5) diabetic + exercise + nano-eugenol (diabetic+exercise+nano model). diabetes was induced in the experimental groups 2 through 5 by the intraperitoneal injection of streptozotocin at 4mg/100 grams of the rats’ body weight. the nano-eugenol supplement was also gavaged into the supplement groups 4 and 5 only. groups 3 and 5 exercised progressively at a speed of 8 to 20 meter/min for 5 to 30 min, five days a week over the 8-week study duration. results: the diabetic rats that exercised and were treated with the nano-eugenol, showed a significant decrease in p38-mapk gene expression compared to the normal model group (p=0.001). the study of the therapeutic modalities also showed that only the diabetic + exercise + nano-eugenol group showed a significant increase in npy and a-rα2a genes compared to the normal model (p=0.001). conclusion: based on the results, the use of nano-eugenol supplementation combined with aerobic exercise is likely to be effective in controlling the neurological damages due to diabetes by negatively regulating the p38-mapk gene while positively regulating the npy and a-rα2a genes in the drg region.