Evaluation of Cytotoxicity Effects of Combination Nanocurcumin and Berberine in Breast Cancer Cell Line

Background: berberine and nanocurcumin are two herbal medicines with strong anticancer effects on tumor cells, but low toxicity on normal cells, when used alone. breast cancer is known as the most common cancer in women and second deadly one. in this study, we evaluated the cytotoxicity effects of combination berberine and nanocurcumin in breast cancer cell line to see whether they have further synergism cytotoxicity on mcf-7 breast cancer cell line.methods: the cytotoxicity effects of berberine and nanocurcumin alone and in combination, were evaluated in mcf-7 cell lines using mtt cytotoxicity test. statistical analysis is done through oneway anova and tukey multiple range tests.results: analyzing results of this study showed that cytotoxicity of nanocurcumin was higher than berberine in a dosedependent manner. the ic50 of combination berberine and nanocurcumin was lower and showed higher cytotoxicity in mcf-7 cells compared with the time we use each of these drugs alone.conclusion: in this study cotreatment of berberine and nanocurcumin significantly inhibited the growth of mcf-7 breast cancer cell line and resulted in synergism cytotoxicity effects. these results indicated on their potency to further combination of these two drugs with other agents and common chemotherapies to improve breast cancer outcomes.