the effect of stem cell factor on proliferation of human endometrial cd146+ cells

Background: stem cell factor (scf) is a transcriptional factor which plays crucial roles in normal proliferation, differentiation and survival in a range of stem cells. objective: the aim of the present study was to examine the proliferation effect of different concentrations of scf on expansion of human endometrial cd146+ cells. materials and methods: in this experimental study, total populations of isolated human endometrial suspensions after fourth passage were isolated by magnetic activated cell sorting (macs) into cd146+ cells. human endometrial cd146+ cells were karyotyped and tested for the effect of scf on proliferation of cd146+ cells, then different concentrations of 0, 12.5, 25, 50 and 100 ng/ml was carried out and mitogensstimulated endometrial cd146+ cells proliferation was assessed by mtt assay. results: chromosomal analysis showed a normal metaphase spread and 46xx karyotype. the proliferation rate of endometrial cd146^+ cells in the presence of 0, 12.5, 25, 50 and 100 ng/ml scf were 0.945 plusmn;0.094, 0.962 plusmn;0.151, 0.988 plusmn;0.028, 1.679 plusmn;0.012 and 1.129 plusmn;0.145 respectively. there was a significant increase in stem/ stromal cell proliferation following in vitro treatment by 50 ng/ml than other concentrations of scf (p=0.01). conclusion: the present study suggests that scf could have effect on the proliferation and cell survival of human endometrial cd146p+p cells and it has important implications for medical sciences and cell therapies.