Resveratrol Supplementation Rescues Pool of Growing Follicles and Ovarian Stroma From Cisplatin-Induced Toxicity on the Ovary in Sprague-Dawley Rats: An Experimental Study

Background: cisplatin is a potent antineoplastic agent for many cancers but causes several levels of gonadal damage. ovarian toxicity is a major concern of young cancer patients undergoing chemotherapy. objective: this study sought to examine the effect of cisplatin and resveratrol supplementation on ovarian function in sprague-dawley rats. materials and methods: in this experimental study, 45 cyclic sprague-dawley rats with an average weight of 160 gr were divided into 9 groups (n=5/group). group 1 was used as control and received distilled water. groups 2 and 9 received cisplatin only. groups 3, 4, and 5 received different doses of resveratrol after a single dose of cisplatin. groups 6, 7, and 8 received resveratrol before cisplatin. at sacrifice, the ovary was analyzed for histopathology, biochemical indices of oxidation and hormonal assay. results: relative and absolute organ weights were notably increased (p=0.001, 0.01) in the prophylactic groups relative to the groups that received resveratrol after cisplatin. also, glutathione, superoxide dismutase and catalase were significantly increased (p=0.047, 0.01, 0.023) in a dose-dependent manner when compared to cisplatin group only. malondialdehyde decreased significantly (p=0.001) in the groups that received high dose resveratrol compared with the control and cisplatin alone groups. although oestrogen showed no significant difference within the groups (p=0.48), resveratrol significantly increased progesterone, follicle stimulating hormone and luteinizing hormone levels (p=0.007, 0.001, 0.006) at high doses when compared with cisplatin alone groups. ovarian histoarchitecture was best preserved in the prophylactic groups in a dose-dependent manner. conclusion: resveratrol supplementation confers protection and preserves ovarian follicles from cisplatin toxicity in sprague-dawley rats.