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The effects of vitrification on spindle apparatus of in vitro matured germinal vesicle in mice
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نویسنده
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Mahmoadi Reza ,Amiri Iraj ,Pasbakhsh Parichehr ,Ragardi Kashani lraj ,Abbasi Mehdi ,Aboulhasani Farid ,Mehrannia Tooba ,Sobhani Aligholi
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منبع
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international journal of reproductive biomedicine - 2008 - دوره : 6 - شماره : 4
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چکیده
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Background: routine oocytes cryopreservation remained an elusive technique in the wide ranges of available assisted reproductive technologies. the micro tubules of oocytes are vulnerable to cry oprotectants and thermal change during cryo preservation,objective: the effects of a vitrification protocol were investigated on the spindle and chromosome configurations of mice oocytes cryopreserved at the germinal vesiclestage.materials and methods: germinal vesicle with cumulus cells were transferred to vitrification solution which was composed of 30% (v/v) ethylene glycol, 18% ficoll-70 and 0.3 m sucrose either by single step or in step-wise way. following vitrification andin vitro maturation (mil), the matured oocytes were immonostained for meiotic spindlesand chromosomes, before visualization using fluorescent microscopy.results: a statistically significant increase was observed in the survival and maturationrate in step-wise vitrification (88.96% and 71.23% respectively) compared to single stepvitrification (70.6% and 62.42% respectively) (p<0.05). normal spindle morphologyafter vitrification-thawing in step-wise vitrification group (77.26%) was higher thansingle step vitrification group (64.24%) but lower than control group (94.75%)(p
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کلیدواژه
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Vitrification ,Germinal vesicle oocyte ,Mice ,Microtubule.
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آدرس
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yasuj university of medical sciences, Faculty of Medicine, Department of Anatomy, ایران, hamadan university of medical sciences, Faculty of Medicine, Department of Anatomy, ایران, tehran university of medical sciences tums, Medicine faculty, Department of Anatomy, ایران, tehran university of medical sciences tums, Faculty of Medicine, Department of Anatomy, ایران, tehran university of medical sciences tums, Faculty of Medicine, Department of Anatomy, ایران, tehran university of medical sciences tums, Faculty of Medicine, Department of Anatomy, ایران, kermanshah university of medical sciences, Faculty of Medicine, Department of Anatomy, ایران, tehran university of medical sciences tums, Faculty of Medicine, Department of Anatomy, ایران
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Authors
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