Determination of effective dosage of FSH and heG in the maturation of preantral follicles and enclosed oocytes in mice

Background: in vitro maturation (ivm) of oocytes reduces the costs and averts the side-effects of gonadotropin stimulation for in vitro fertilization (ivf). reliable ivm is an intellectual, scientific and clinical challenge with a number of potential applications.objective: the effect of hcg was evaluated on the timing and regulation of in vitro ovulation for the syrian mice oocytes in the presence and absence of fsh. materials and methods: preantral follicles, isolated from the ovaries of 6 weeks-old mice, were cultured in tcm-199 medium. the effect of 10-200 mll.l/ml fsh and 1.5 iv/m) hcg was seen on the follicle maturation, as well as the changes in ovulationcapacity of enclosed oocytes, after the incubation period of 6 days at 37°c, 92% humidity and 50/0 co2 in air. results: 100 miv/ml fsh showed increased follicle diameter, survival, germinal vesicle breakdown (gvbd) and oocyte maturation rates (p