the impact of ovarian stimulation and luteal phase support on embryo quality and implantation process in mice

Background: the luteal phase defect is a common event following the ovarian stimulation.the aim of the present study was to evaluate the use of human chorionic gonadotropine (hcg)and progesterone hormones to improve the luteal phase defect.materials and methods: 60 mice were superovulated routinely with human menopausalgonadotropin (hmg) (7.5u) and hcg (10u). the mice were mated and divided into 3groups: 1- control (n=20) 2- hcg treatment (n= 20), and 3-progesterone treatment (n=20).each group was divided again into two subgroups. the mice (10 from each group) had noinjection in group one and were injected intraperiteneal (ip) by hcg (5u/day) andprogesterone (1mg/day) subcutaneously (sc) in groups 2 and 3, respectively for four days. onthe day 5, the animals were killed by cervical dislocation and the uterus were flushed to countthe number of blastocyst and their quality. the above treatment were carried out for 12 daysin the other 10 mice in each group. similarly group one had no injection and groups 2 and 3were injected by hcg and progesterone for 12 days respectively by the same manner asmention above. the animals were killed on day 13 and the implanted embryos were counted.the uterus and ovary were processed on days 5 and 13 of pregnancy for histological studies.results: the mean number of blastocysts per mouse were: 12.2%, 2.6% and 3% in group 1 to3, respectively. the nomber of implanted embryos were 29 as: 13 living fetus in one mouseand 16 resorption fetus in the other. the morphology of uterus on day 5 was as follow: nodevelopment in the stroma and endometrial gland in control group, the stroma andendometrial gland so developed to form the saw teeth appearance which indicated onreceptivity of uterus in hcg treated group similar to progesterone treated group, but withoutthe saw teeth appearance. the continuation of hcg injection maintained the receptivity ofuterus; while, the continuation in progesterone caused metaplesia of epithelium. themorphology of ovaries in all three groups showed no changes in corpus luteum size on day 5,and showed the following changes on day 13: increasing the number of primary andsecondary follicles in control group; while, reducing the size of corpus luteum in hcg group.conclusion: progesterone did not improve the uterus and implantation rate. the prolongedusage of progesterone can change the morphology of uterus to more abnormal state inconterast to the prolonged usage of hcg.