the in-vitro effects of nicotine, cotinine and leptin on sperm parameters analyzed by casa system

Backgrand: cotinine (cot) is a major degradation product of nicotine (nic). theparticipation of leptin in female reproduction is well-established, but any role inmale reproductive function is at the best tenuous.objective: the aim of this study was to evaluate the in-vitro effects of nicotine,cotinine and leptin on sperm parameters in normal semen of non-smokers fertilemen.materials and methods: ten healthy nonsmokers aged 25-40 years old weredevided into 7 groups, thier semens were divided into 7 aliquots. (a) was layeredwith basal solution 70 ng/ml nic, (b) 35 ?g/ml nic, (c) 300 ng/ml cot, (d) 200?g/ml cot, (e) 30 ng/ml leptin, (f) 300 ng/ml leptin respectively and (g) waslayered with mhtf. after migration, the samples were examined at time 0, +1, +2,+4, +8, and + 24 h of incubation.results: these findings were obtained: sperm count: 75.66±66.25x10 6 /ml, forwardmotility: 75.55±14.80%, progress: 33.66±13.01, vsl: 51.58±6.99 ?m/s, vcl:103.33±14.52 ?m/s, alh: 4.33±0.77 ?m, bcf: 25.60±2.97 hz, str: 79.33±8.04%, lin: 52.55±10.52 %, elo: 74.22 ± 12.76 % and are: 3.04 ± 1.50 u/sq. theparameters were similar before 8 hr and were being decreased after that.conclusion: according to the results. nicotin and cotinin have negative effects onthe sperm parameters but despite the positive effect of leptin, there is no correlationbetween leptin concentration in semen and its physical characteristics.