Cytogenetic analysis in couples with recurrent spontaneous abortion

Background: recurrent abortion is a difficult medical problem happening in about 1-2% of fertilewomen. most spontaneous miscarriages which happen in the first and second trimesters are caused bychromosomal abnormalities.objective: the present study tries to find the rate of chromosomal abnormalities in couples withrecurrent pregnancy loss.materials and methods: in total 165 couples were referred to genetic counselling clinic with a history ofat least three previous abortions. in all women antibodies against toxsoplasmose, rubella andcytomegalovirus (cmv) were analysed by eliza. in 88 couples karyotyping was conducted by analysisof g and/or c banding. metaphase spreads were made from phytohaemaglutinin-stimulated peripherallymphocytes using standard cytogenetic techniques. the chromosomal status was analyzed usingcytovision ultra ver.4.0 from applied imaging.the x2-test and anova were used for statistical evaluation. the level of p<0.05 was considered assignificance.results: most of the patients had 3 repeated abortions (61.2%). cytogenetic analysis performed for 88couples and karyotypes of 12.50/0 of them were abnormal. the majority of them had monosomy x(6.82%), followed by balanced translocation (2.27%). the number of female carries chromosomalabnormality exceeded significantly than of male. coefficient of inbreeding in more than 50% of coupleshadfifth degree of relationship (89 out of 165).conclusion: our results showed that 12.50/0 of the couples with missed abortion had an abnormalkaryotype, with no other abnormality, cytogenetic findings in spontaneous aborted specimens couldprovide valuable information for genetic counseling