|
|
|
|
The effect of mouse embryonic fibroblast in direct differentiation of mouse embryonic stem cells
|
|
|
|
|
|
|
|
نویسنده
|
Hashemi-Tabar Mahmoud ,Javadnia Fatemeh ,Orazizadeh Mahmoud ,Sakei Ghasem ,Baazm Maryam
|
|
منبع
|
international journal of reproductive biomedicine - 2007 - دوره : 5 - شماره : 1 - صفحه:29 -33
|
|
چکیده
|
Background: since embryonic stem (£s) cells have the dual ability to proliferate indefinitelyand differentiate into multiple tissue types, es cells could potentially provide an unlimitedcell supply for human transplantation.objective: in order to study the differentiation of mouse embryonic stem (mes) cells, theywere cultured in suspension by using es media without leukemia inhibitory factor (lif) toinduce spontaneous differentiation. cellular morphology of differentiated derivatives wasthen evaluated.materials and methods: undifferentiated mes from our laboratory were cultured in threedifferent settings by using es media containing 0.1% / 1mm trypsin/edta and removinglif; in the absence of murine embryonic fibroblast (mef) feeder cells (group 1), in thepresence of mef feeder cells with a density of 0.5
|
|
کلیدواژه
|
Embryonic stem cells ,Embryoid bodies ,Mouse embryonic fibroblast ,Coculture. Differentiation
|
|
آدرس
|
ahvaz jundishapur university of medical sciences, School ofMedicine, Department of Anatomy, ایران, ahvaz jundishapur university of medical sciences, School ofMedicine, Department of Anatomy, ایران, ahvaz jundishapur university of medical sciences, School ofMedicine, Department of Anatomy, ایران, ahvaz jundishapur university of medical sciences, School ofMedicine, Department of Anatomy, ایران, ahvaz jundishapur university of medical sciences, School ofMedicine, Department of Anatomy, ایران
|
|
پست الکترونیکی
|
hashemi_tabar@hotmail.com
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|