The Effect of Hyaluronic Acid on Motility, Vitality and Fertilization Capability of Mouse Sperms After Cryopreservation

Background: various approaches have been used in the attempts to improve the qualityof frozen.thawed mouse sperms. according to literatures, it seems that hyaluronic acid(ha) has an important role on the permeability and motility of sperms and theirinteraction with gametes.objective: for evaluation of ha supplementation on sperm characteristics andfertilization capability, we investigated the effect of different doses of ha on mousesperm morphology, motility, vitality and fertilization capability after freezing andthawing.materials and methods: the cauda epididymes was removed from 6 male mice withaseptic method. the sperm samples were frozen in 1.8 ml cryotubes with 18% raffmoseand 3% skimmed milk containing cryo-protectant solution. ha at the concentration of750 , 1000 or 1250 ug/ml was supplemented to frozen-thawed sperms. sperm motilitywas measured with microscope, and fertilization rate was evaluated after routine ivf bycounting the fertilized oocytes. for sperm morphology, papaniclau staining was usedwhile; eosin b was used for the assessment of sperm viability rate.results: ha supplementation (750 ug/ml) improved motility parameters (p < 0.05) andincreased the fertility rate (p < 0.05). the effect of 1,000 ug/rnl ha was also positive onthe sperms. but 1,250 ug/rnl ha had negative effect on above mentioned characteristic.on the other hand, none of these doses had any effect on sperm morphology.conclusion: the dose of 750 ug/ml of ha has the greatest effect on the motility,vitality and fertility rate of sperms after cryopreservation.