Effects of melatonin on histopathological changes after experimental ovarian torsion-detorsion in cat

Background: during the detorsion of a torsioned ovary, oxidant agents are released andmelatonin as an antioxidant can reduce ischemia. we studied the histopathologicalchanges after using melatonin on experimental torsioned ovary in cat.objective: the aim of this experimental study was to investigate the effects ofmelatonin on histopathological changes in torsion - detorsion injury in cat ovary.materials and methods: an adnexal torsion - detorsion model was created by using 20adult cats randomly divided equally in to 2 groups of saline and melatonin. ischemia wasinduced by iathrogenic 360 0 clockwise torsion of the cat adnex for 3 hr. reperfusionwas achieved for 3 hr. melatonin or saline were injected intra peritoneally (1omglkg) 30min before ovarian detorsion in both groups. after 3 hr of ovarian detorsion, ovariantissue was removed and fixed in 10% formalin solution, embedded in paraffm andevaluated for ischemic indices.results: histological examination showed a significant improvement in ovarianmorphology in the melatonin treated cats. edema and vasoconstriction in saline groupwere more severe than melatonin group (p-value = 0.009). hemorrhage and leukocyteinfiltration were also more obvious in saline group (p-value 0.0018)conclusion: our results demonstrated that melatonin administration reduced ovarianhistopathological damage due to oxidative injury associated with torsion.