In vitro maturation media, cysteamine concentration and glutathione level affect blstocysts development in mouse

Background: preparation of oocytes is one of the critical factors that determine thedeve lopmental competence of embryos produced by in vitro fertilization (ivf).object ive: in this study, the effect of cysteamine, type of media and glutathione (gsh)level on blastocysts development after in vitro maturation of mouse oocyte s wereinvestigated.materials and methods: premature female mice were primed with pregnant marestimulating gonadotrophin (pmsg), and germinal vesicle (gv) stage oocytes wereobta ined 45 hr later. gv oocytes were cultured in presence of 0, 50, 100, 200 and 500urn cysteamine in tcm199 and meme media. after ivm, mil oocytes were in vitrofertilized (ivf) and in vitro cultured (ivc) in order to observe embryo development. agroup ofln vivo ovulated (iva ) oocytes after priming with pmsg and hcg also wereincluded in this study. 5.5-dithio-bis (2nitrobenzoic acid) dtnb-recycling protocolwas used for gsh assay.results: rate of ivm and ivf were improved in all oocytes treated with cysteamin inthe two medium except 500 urn (81% mil rate in tcm and 64% mil in meme). rateof lastocyst in 100 urn cysteamine in tcm 1199 and 200 urn in meme was highercompared to control groups (in tcm 45% and in meme 35%). in vivo mil and gvoocytes represented the highest and lowest gsh level respectively.conclusion: our results revealed that the media and concentration of cysteamine canaffects on ivm. ivf and rate of biastocysts development on dose depen dant manner