Non-invasive evaluation of embryo morphological plasticity by designing of new transgenic gene cassette

Background: determination of transgenic embryos from non transgenic embryos sibling is animportant step in producing homozygous transgenic mice. these steps need by pcr or southernblotting followed extraction of dna, but both techniques require skill and consume time.objective: the aim of this study was simulation of high accuracy method using novelenhanced green fluorescent protein (egfp) gene cassette to eliminate some consumetime in livestock industry to assay high quality embryos and morphological plasticity.materials and methods: we modified pqe-tri systemic vector with egfp and iressequence to trace out coming planning of molecular farming transgene using coinjectionmethod.results: the combination of these sequences successfully showed the faint and normalexpression of transgene in mouse pre-implantation stage embryos. the low rate ofsurviving green positive embryos as compare as only medium and physical treatmentscould be partly from the physical damage caused by microinjection and gene integration.furthermore, application of the enhanced gfp marker facilitated subtle detection ofasymmetrical division appeared in some of transgenic embryos.conclusion: the results of current mouse simulation model imply that an efficientproduction and propagation of transgenic livestock can be done by co-injection of everyeconomical gene with this novel transgene and also it can be suitable gene cassette fornumerous experiments and study of protein behaviors in living cells.