Colony formation ability of frozen thawed spermatogonial stem cell from adult mouse

Background: the basis of spermatogenesis is the spermatogonial stem cells (sscs).the concentration of sscs is very small. however, a system that supports theproliferation and maintenance of sscs in vitro could be used to preserve and expandsscs numbers as well as increase success in transplantation. it is a new avenue torestore spermatogenesis in azoospermia subjects.objective: proliferation and enhancement of frozen-thawed sscs numbers during in vitroculture.materials and methods: both sertoli and spermatogonial cells were isolated fromadult mouse testes. frozen-thawed spermatogonial cells were cultured in two groups: simpleculture (experimental 1) and co culture with sertoli cells (experimental 2). also, fresh cellswere considered as control groups: simple culture (controli) and co culture with sertoli cells(control 2).assay of the spermatogonial-cell-derived colonies was carried out at the endof each week.results: results indicated that the viability rate of the frozen cells after thawing(68.4± i 0.2%) was influenced by cryopreservation procedure significantly (p ::::0.00 i). inaddition, the number of the colonies and their diameters in the co-culture system withfresh cells (25.1±5.2 and 205 .8±50 urn, respectively) were more than other groups andthe differences were significant (p