Association of plasminogen activator inhibitor-1 and angiotensin converting enzyme polymorphisms with recurrent pregnancy loss in Iranian women

Background: recurrent pregnancy loss (rpl) defined by two or more failedpregnancies before 20 weeks of gestation. several factors play a role in rplincluding thrombophilic conditions which can be influenced by genepolymorphisms. plasminogen activator inhibitor-1 (pai-1) and angiotensinconverting enzyme (ace) genes are closely related to fibrinolytic process,embryonic development and pregnancy success.objective: the aim of this study was to investigate the relationship between rpl and common polymorphisms in ace and pai-1 genes.materials and methods: in this case control study, 100 women with recurrent abortions (at least two) were selected as cases and 100 healthy women with two or more normal term deliveries without a history of abortion as controls. total genomic dna was isolated from blood leukocytes. the status of the pai-1 4g/5g and ace (d/i) polymorphism was determined by pcr-rflp.results: homozygosity for pai-1 4g polymorphism was seen in 17 cases (17%), and 5 controls (5%) (p=0.006) so patients with homozygote 4g mutation were significantly more prone to rpl in contrast to control group (or: 4.63, % 95 ci: 1.55-13.84). in addition, 7 patients (7 %), and no one from the control group, were homozygote (i/i) for ace polymorphism (p=0.034), suggesting no significant associations between ace d allele or dd genotype and rpl.conclusion: considering these results, because 4g/4g polymorphism for pai-1gene could be a thrombophilic variant leading to abortion, analysis of this mutationand other susceptibility factors are recommended in patients with rpl.