The Viability of Mouse Spermatogonial Germ Cells on A Novel Scaffold, Containing Human Serum Albumin and Calcium Phosphate Nanoparticles

Background: in spermatogenesis, spermatogonial cells differentiate to the haploidgametes. it has been shown that spermatogenesis can be done at in vitro condition.in vitro spermatogenesis may provide an open window to treat male infertility.objective: the aim of this study was to evaluate the effects of a novel scaffoldcontaining human serum albumin (hsa)/tri calcium phosphate nanoparticles (tcpnps) on the mouse spermatogonial cell line (scl).materials and methods: first, tcp nps were synthesized by reaction of calciumnitrate and diammonium phosphate at ph 13. then, serial concentrations of tcpnps were separately added to 500 mg/ml hsa, and incubated in the 100oc waterfor 30 min. in the next step, each scaffold was cut (2×2mm), placed into sterile wellof microplate, and then incubated for 1, 2, and 3 days at 37oc with mouse scl.after incubation, the cytotoxicity of the scaffolds was evaluated by different testsincluding 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (mtt)assay, lactate dehydrogenase (ldh) assay, vital staining, and cell counting. on theother hand, the release of tcp nps and hsa from the scaffolds was measured.results: based on microscopic observation, the size of cavities for all scaffolds wasnear 200-500 ?m, and the size of tcp nps was near 50-100 nm. all toxicity testsshowed that the increase of tcp concentration in the scaffold did not affect mousescl. it means that the percentage of cell viability, ldh release, vital cells, and cellquantity was 85%, 105%, 90%, and 110%, respectively. but, the increase ofincubation time led to increase of ldh release (up to 115%) and cell count (up to115%). also, little decrease of cell viability and vital cells was seen when incubationtime was increased. here, no release of tcp nps and hsa was seen after increaseof tcp concentration and incubation time.conclusion: it can be concluded that the increase of tcp concentration in hsa/tcp nps scaffold does not lead to cytotoxicity. on the other hand, the increase ofincubation time leads to increase of mouse scl cell death. in this study, it wasfound that tcp nps and hsa could not release from the scaffolds. in future, bothproliferation and differentiation of mouse scl on hsa/tcp nps scaffold must bechecked over more wide incubation times.